Research Flow & Imaging
Cytometry Resource








Natasha Barteneva
PhD, MD, Department of Pediatrics, Harvard Medical School
Faculty Associate, Harvard Microbiology Initiative

The development of novel cytometric and imaging methods to analyze heterogenous cell populations, microorganisms and microvesicles
Flow cytometry and Imaging flow cytometry is rapidly evolving field. We are collaborating with biomedical and bioengineering scientists in the variety of directions -to develop instrument platforms for the detection and sorting of micro- and nanoparticles, to improve diagnostics of cancer, to evaluate biodiversity of phytoplankton etc.

Microvesicles and intercellular communication
A second line of investigation involves the mechanisms of intercellular interaction mediated through microvesicles (MVs). There is a rapidly growing body of evidence that production of MVs is a universal feature of cellular life. MVs can incorporate microRNA (miRNA), mRNA, mtDNA, DNA and retrotransposons, camouflage viruses, viral components from immune surveillance, and transfer cargo between cells. These properties make MVs an essential player in intracellular communication. We are interested in the mechanisms of their production and contribution to cell:cell and pathogen:cell interaction, and intercellular signaling.  We are also developing methods that allow a quantitative analysis of pathogen-derived and cell-derived microvesicles given their potential as circulating biomarkers.

Selected Publications:
Barteneva NS*, Maltsev N, Vorobjev IA. Microvesicles and intercellular communication in the context of parasitism. Front Cell Infect Microbiol. 2013; 3:49.
Mantel PY, Hoang AN, Goldowitz I, Potashnikova D, Hamza B, Vorobjev I, Ghiran I, Toner M, Irimia D, Ivanov AR, Barteneva N, Marti M. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe. 2013 May 15; 13(5):521-34.
Barteneva NS*, Fasler-Kan E, Bernimoulin M, Stern JN, Ponomarev ED, Duckett L, Vorobjev IA. Circulating microparticles: square the circle. BMC Cell Biol. 2013; 14(1):23.
Sotnikov I, Veremeyko T, Starossom SC, Barteneva N, Weiner HL, Ponomarev ED. Platelets recognize brain-specific glycolipid structures, respond to neurovascular damage and promote neuroinflammation. PLoS One. 2013; 8(3):e58979.
Barteneva NS*, Ketman K, Fasler-Kan E, Potashnikova D, Vorobjev IA. Cell sorting in cancer research-Diminishing degree of cell heterogeneity. Biochem Biophys Acta, Reviews on Cancer, 2013 Aug; 1836(1):105-22.
Fasler-Kan E, Barteneva NS*, Ketterer S, Wunderlich S, Reschner A, Nurzhanova A, Flammer J, Huwyler J, Meyer P. Human cytokines activate JAK-STAT signaling pathway in porcine ocular tissue. Xenotransplantation, 2013, In Press.
Ghosh SK, Yigit MV, Uchida M, Ross AW, Barteneva N, Moore A, Medarova Z. Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering RNA nanodrug demonstrates efficacy in models of adenocarcinoma. Int J Cancer. 2013 Oct 1.

Vorobjev IA, Buchholz K, Prabhat P, Ketman K, Egan ES, Marti M, Duraisingh MT, Barteneva NS*. Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Malar J. 2012; 11:312.
Barteneva NS*, Fasler-Kan E, Vorobjev IA. Imaging flow cytometry: coping with heterogeneity in biological systems. J Histochem Cytochem. 2012 Oct; 60(10):723-33.

Ponomarev ED, Veremeyko T, Barteneva NS*. Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry. BMC Res Notes. 2011; 4:517.
Ponomarev ED, Veremeyko T, Barteneva N, Krichevsky AM, Weiner HL. MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-a-PU.1 pathway. Nat Med. 2011 Jan; 17(1):64-70.
Kovalenko EI, Ranjbar S, Jasenosky LD, Goldfeld AE, Vorobjev IA, Barteneva NS*. The use of HaloTag-based technology in flow and laser scanning cytometry analysis of live and fixed cells. BMC Res Notes 2011, 4: 340.
Khoudoleeva O, Gretsov E, Barteneva N, Vorobjev I. Proliferative index and expression of CD38, Zap-70 and CD25 in different lymphoid compartments of chronic lymphocytic leukemia patients. Pathology and Lab Medicine Inter 2011, 3: 7-16.

Fasler-Kan E, Suenderhauf C, Barteneva N, Poller B, Gygax D, Huwyler J. Cytokine signaling in the human brain capillary endothelial cell line hCMEC/D3. Brain Res. 2010 Oct 1; 1354:15-22.
Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA. Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology. PLoS One. 2010; 5(3):e9625.
Barteneva N*, Vorobjev I. Quantum dots in microscopy and cytometry: immunostaining applications. In: Microscopy: Science, Technology, Applications and Education, 2010, Eds A. Mendez-Vilas and J. Diaz, vol 4, pp 710-721.Fasler-Kan E, Barteneva N, Ketterer S, Wunderlich K, Huwyler J, Gygax D, Flammer J, Meyer P. Activation of the JAK-STAT intracellular pathway in human retinal pigment epithelial cell line ARPE-19. Int J Interferon Cytokine Med Res 2010, 2: 127-136.


Bernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD. Differential stimulation of monocytic cells results in distinct populations of microparticles. J Thromb Haemost. 2009 Jun; 7(6):1019-28.
Ahmed F, Friend S, George TC, Barteneva N*, Lieberman J. Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry. J Imm Methods. 2009 Aug 15; 347(1-2):79-86.
Barrington RA, Schneider TJ, Pitcher LA, Mempel TR, Ma M, Barteneva NS, Carroll MC. Uncoupling CD21 and CD19 of the B-cell coreceptor. Proc Natl Acad Sci U S A. 2009 Aug 25; 106(34):14490-5.
Yew JY, Wang Y, Barteneva N, Dikler S, Kutz-Naber KK, Li L, Kravitz EA. Analysis of neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry. J Proteome Res. 2009 Mar; 8(3):1271-84.

Medarova Z, Kumar M, Ng SW, Yang J, Barteneva N, Evgenov NV, Petkova V, Moore A. Multifunctional magnetic nanocarriers for image-tagged SiRNA delivery to intact pancreatic islets. Transplantation. 2008 Nov 15; 86(9):1170-1177
Muskavitch MA, Barteneva N, Gubbels MJ. Chemogenomics and parasitology: small molecules and cell-based assays to study infectious processes. Comb Chem High Throughput Screen. 2008 Sep; 11(8):624-46.
Maresz K, Ponomarev ED, Barteneva N, Tan Y, Mann MK, Dittel BN. IL-13 induces the expression of the alternative activation marker Ym1 in a subset of testicular macrophages. J Reprod Immunol. 2008 Jul; 78(2):140-8.
Stern JN, Keskin DB, Barteneva N, Zuniga J, Yunis EJ, Ahmed AR. Possible role of natural killer cells in pemphigus vulgaris - preliminary observations. Clin Exp Immunol. 2008 Jun; 152(3):472-81


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