Research Flow & Imaging
Cytometry Resource


 
 
Instrumentation
 

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TECHNOLOGY


The Research Flow and Imaging Cytometry Resource at Boston Children's Hospital (Program in Molecular and Cellular Medicine) is equipped with unique instrumentation that allows our investigators to perform cutting edge research in cytometry, imaging cytometry and molecular imaging.


EQUIPMENT
MOLECULAR IMAGERS (IN VIVO)

Mice Imaging


An IVIS SPECTRUM was recently acquired with the NIH Shared Instrumentation grant (scientific team led by Judy Lieberman). The IVIS Spectrum is one of the most versatile and advanced imaging systems available with tomographic capabilities. It has an incredibly sensitive camera combined with high energy trans and epi illumination that allows for bioluminescence and fluorescence detection. The system has 3D tomographic software and spectral umixing capabilities. Location: the NRB0044 room at the Harvard Center for Comparative Medicine.

Ivis Spectrum


CELL SORTING INSTRUMENTATION

The 5-laser, 20-parameter FACSAria 2-SORP (lasers: 355 nm, 488 nm, 633 nm, 405 nm, 594 nm) is equipped with a PMT option for FSC, enhanced optics and digital focusing and a unique 300 mW 488 nm laser from Coherent. It also has the unique option of changing sorting needles (to adjust core size for nanoparticle sorting). This instrument was developed by BD engineers for sorting small microparticles. It is available to investigators during working hours (by preliminary arrangement, after hours for advanced users) after initial training and passing a sorting test. Location: the Armenise Building, 2nd Floor, room 231 (210 Longwood Ave.)

Cell Sorter


FACSAria-BL2+ is a 4-laser, 13-parameter for flow sorting applications (lasers: 488 nm, 633 nm, 405 nm, 651 nm). The machine is equipped with a sample cooling system and has 2- and 4-way sorting options into 1, 3 and 15 ml tubes, as well as an Automated Cell Deposition Unit for microplate and slide sorting. The flow core designed the Aria custom installation inside a BL2 + level biohazard containment cabinet with an internal Aerosol Management System and a separate remote system control workstation located outside of the BL2 + Lab which provides the ability to safely sort potentially suspect or known biohazardous samples. The instrument is operated only by highly-experienced operators. Location: BL2+ block, next to room 239.

Cell Sorter 2


The FACSAria-STANDARD (lasers: 488 nm, 633 nm, 405 nm) is equipped with a sample cooling system and has 2- and 4-way sorting options into 1, 3 and 15 ml tubes, as well as an Automated Cell Deposition Unit for microplate and slide sorting. the instrument  is available to investigators on a 7/24 basis via an on-line schedule after initial training and passing a sorting test. Location: room 273A, 2nd Floor, in the Warren-Alpert Building.


BENCHTOP FLOW CYTOMETRY ANALYSERS

There are two FASCSCanto2 (BD Biosciences) digital multi-color cytometers that have standard 3 lasers-configuration (405 nm, 488 nm and 633 nm) and provide 8-color and 10 parameter analytical capabilities. FACSCanto-WAB – is located on the 1st Floor; and the FACSCanto-CLSB – is located on the 3rd Floor, in room 3034.


The FACSCalibur is a four color, six parameter non-sorting bench top instrument capable of performing four color data acquisition and analysis. It is equipped with 488nm and 633 nm lasers. Location: in the Armenise Building, on the 2nd Floor. Instruments are available to investigators on a 7/24 basis via an on-line schedule after initial training.


IMAGING CYTOMETERS

The Imagestream100 (Amnis-Millipore) is a hybrid instrument combining the statistical power of flow cytometry and visual power of microscopy. The unique software program IDEAS allows it to perform robust, multiparameter quantitative analyses and generate  brightfield, darkfield and fluorescence images of every cell. Graphics, statistics, images, and other data components can be easily copied to the Windows clipboard. The instrument implements the TDI (Time Delay Integration) System that greatly increases sensitivity of acquisition. The images' detection bands are fixed (470-500 nm; 400-470 nm; 500-560 nm; 560-595 nm; 595-660 nm; 660-735 nm). The system is compatible with a wide variety of fluorochromes and fluorescent proteins used in flow cytometry and microscopy.

 

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The Program in Cellular and Molecular Medicine (PCMM) is an affiliate of Children's Hospital Boston and Harvard Medical School.