The Flow and Imaging Cytometry Resource (FICR) at the Immune Disease Institute, a Harvard Medical School affiliate and recent affiliate with the Children’s Hospital, Boston, provides institute investigators with state-of-the-art equipment along with human expertise to support cell sorting (separation), acquisition, and analysis of flow and imaging cytometric data in several locations within the Immune Disease Institute.
For cell sorting, the FICR is equipped with two BD FACSAria2 (20- and 11-parameters) and FACSVantage-Diva Cell Sorters from BD Biosciences. For analysis purposes, the FICR is equipped with 8-color, 11-parameters FACSCanto2 and two basic FACSCaliburs. For experiments in Imaging Cytometry the Resource hasa 3-laser imaging cytometer Imagestream 100 (Amnis) as well as access to a laser scanning cytometer iCys (Compucyte). After training, the analyzers and 20-parameter sorter are available for use directly by the investigators. The 11-parameters FACSAria IIU is located at the BL2+ lab and is intended for infectious sorts and not-fixed human cells (with accordance of biosafety rules). We are offering comprehensive training, consultation for maximizing the efficiency of the experiments and collaborative development of new methods and applications.